Analysis Note

homogeneous by SDS-PAGE

General description

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel's viper venom. Hydrolyzes peptide and ester bonds at the carboxyl group of arginine. Specifically catalyzes the activation of prothrombin to thrombin. Prothrombin activation is accelerated by Factor Va, phospholipids, and Ca²⁺. Factor Xa was prepared from homogeneous Factor X, using Russels′ viper venom (RVV), which was removed after activation. Inhibited by AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and soybean trypsin inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Native coagulation factor Xa from human plasma prepared from homogeneous Factor X by activation with Russel's viper venom. The venom is removed after activation. During coagulation, in the presence of Ca²⁺ and membrane phospholipids, a complex of Factor Xa and Factor Va (the prothrombinase complex) activates prothrombin to thrombin. Inhibitors include AEBSF (Cat. No. 101500), DFP (Cat. No. 30967), PMSF (Cat. No. 52332), and Soybean Trypsin Inhibitor (Cat. No. 650357). Sold on the basis of plasma equivalent units of Factor X. 100 plasma equivalent units are equal to 800 µg of Factor X.

Legal Information

Chromozym is a registered trademark of Pentapharm AG, Basel

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Heeb, M.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 2728.Lindhout, T., et al. 1994. Biochem. J.297, 131.Morishita, H., et al. 1994. Thrombosis Res.73, 193.Lupu, C., et al. 1993. Thrombosis Haemostasis70, 579.Jordan, S.P., et al. 1992. Biochemistry31, 5374.Schoen, P., et al. 1992. Br. J. Haemotol.81, 255.Tracy, P.B., et al. 1992. Methods Enzymol.215, 329.Lawson, J.H., and Mann, K.G. 1991. J. Biol. Chem.226, 11317.Tans, G., et al. 1991. J. Biol. Chem.266, 21864.Owen, W.G., et al. 1974. J. Biol. Chem.249, 594.

Packaging

10 u in Plastic ampoule

Physical form

Lyophilized from 700 mM NaCl, 20 mM Tris-HCl, pH 7.4.

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol CHROMOZYM^® X substrate per min at 25°C.

Warning

Toxicity: Standard Handling (A)